Study on the inhibition of cell proliferation and the expression of PCNA gene of human glioma U251 by elemene
- VernacularTitle:榄香烯抑制人脑胶质瘤U251细胞增殖和PCNA基因表达的研究
- Author:
Jinhua ZHANG
;
Fenghui YOU
;
Weizhong YANG
;
Chunmei CHEN
;
Chonghong CHEN
- Publication Type:Journal Article
- Keywords:
elemene;
glioma;
proliferation;
PCNA
- From:
Chinese Pharmacological Bulletin
2003;0(10):-
- CountryChina
- Language:Chinese
-
Abstract:
Aim To investigate the inhibition of cell proliferation and the expression of PCNA gene of human glioma U251 by elemene.Methods Normal control and treatment group with elemene in human glioma U251 were studied, then the inhibition effect was examined by MTT methods and the density of half death was worked out (IC50). The expression of PCNA protein and gene was examined by immunohistochemical method and RT-PCR in different action time or at different concentrations.Results Elemene had a marked antineoplastic effect on glioma U251 with the dependence of dose and time,IC50 was 0.062 g?L-1. Elemene exhibited the expression of PCNA protein and gene,which could be examined by immunohistochemical method and RT-PCR. The expression of PCNA mRNA was lowered with the increase of drug concentration and action time.Conclusion Elemene inhibited the expression of PCAN gene,induced the inhibition of cell proliferation,and had a marked antiproliferative effect on glioma cells.
