Antitumor mechanism of HG251,a novel anthracene derivative,in K562/DOX leukemia cells
- VernacularTitle:新型蒽醌类衍生物HG251诱导人白血病K562/DOX细胞凋亡的机制
- Author:
Zhongquan ZHANG
;
Mei XU
;
Songqiang XIE
;
Guoqiang HU
;
Biansheng JI
- Publication Type:Journal Article
- Keywords:
anthracene derivative;
apoptosis;
caspase;
mitochondrial membrane potential;
multidrug resistance
- From:
Chinese Pharmacological Bulletin
2003;0(10):-
- CountryChina
- Language:Chinese
-
Abstract:
Aim To evaluate the mechanism of HG251-induced apoptosis in K562/DOX cells.Methods Cell viability was assessed by MTT assay;cell cycle distribution,apoptosis and mitochondrial membrane potential were measured by flow cytometry;the protein expressions of P-gp,caspase-3,caspase-8,caspase-9,p53,Bcl-xL and cytochrome c in the K562/DOX cells were evaluated by Western blot.Results HG251 was able to inhibit cells proliferation,induce apoptosis,lose mitochondrial membrane potential,activate caspase-3,caspase-8,caspase-9,up-regulate p53 protein and down-regulate Bcl-xL protein in a dose-dependent manner but it had no effect on P-gp protein expression.Conclusion HG251 could overcome apoptotic resistance via up-regulating p53 protein and down-regulating Bcl-xL protein.In addition,HG251 also induced K562/DOX cells apoptosis via mitochondrial pathway and membrane death receptor pathway.
