Inhibition of cardiac hypertrophy by mitofusin 2 in neonatal rat cardiomyocytes
- VernacularTitle:线粒体融合蛋白Mfn2对心肌细胞肥大的抑制作用
- Author:
Chunlei CHEN
;
Tao SHEN
;
Ming ZHENG
;
Yanhong GUO
;
Xiaojun ZHU
;
Guanghui CHEN
- Publication Type:Journal Article
- Keywords:
Mitofusin 2;
Cardiomegaly;
Myocardium;
Cell,cultured
- From:
Journal of Peking University(Health Sciences)
2003;0(05):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To study the expression and function of Mitofusin 2(Mfn2)in cultured neonatal rat cardiomyocytes during PE-induced hypertrophy.Methods:The hypertrophy neonatal rat cardiomyocytes model was induced by 0.01 mol/L PE.RT-PCR and Western blot were applied to assess Mfn2 mRNA expression and protein level respectively.Cultured neonatal rat cardiomyocytes were treated by PE after Ad GFP or Ad Mfn2 infection,the protein synthesis was determined by 3H-leucine incorporation assay.Results:PE led to ANF mRNA level(by~1 fold,P
