Expression of major histocompatibility complex class Ⅱ antigens on lungs in rat with bleomycin-induced pulmonary fibrosis
- VernacularTitle:Ⅱ类主要组织相容性抗原在博来霉素致大鼠纤维化肺组织中的表达
- Author:
Lianfang NI
;
Zhigang ZHANG
;
Dingfang BU
;
Xinmin LIU
- Publication Type:Journal Article
- Keywords:
Major histocompatibility;
Trans-activators;
Pulmonary fibrosis;
Bleomycin;
Rats
- From:
Journal of Peking University(Health Sciences)
2003;0(05):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To explore the expression levels of MHCⅡ molecules and its regulator genes CⅡTA on bleomycin-induced pulmonary fibrosis in rats,and to investigate the underlying immunologic mechanisms of pulmonary fibrosis.Methods:The rats were treated with either a single intratracheal bleomycin injection(fibrosis group)or a normal saline injection(control group).The pathologic changes of lung tissues stained with HE and Masson were observed,and the contents of hydroxyproline were detected on the 7th and 28th day respectively after bleomycin administration.The expression of MHCⅡ molecules in the lung tissues was evaluated with immunohistochemistry techniques,and the percentage of MHCⅡ+ cells was measured.The amounts of total CⅡTA and typeⅠ,Ⅲ and Ⅳ CⅡTA mRNA of lung tissues were measured by real-time PCR using Taqman probe.Results:(1)The percentage of MHCⅡ+ cells in lung tissues increased significantly in fibrosis group compared with that of control group on the 7th day and the 28th day[(0.10?0.03)vs(0.06?0.02),P0.05];In fibrosis group,type Ⅳ CⅡTA mRNA was 667.3% [(2.98?0.92)vs(0.39?0.15),P
