Transfection of human hyperplasia suppressor gene and hyperthermia have a synergistically apoptotic effect on colon cancer cell HT-29 in vitro
- VernacularTitle:人增殖抑制基因(hHSG)联合热处理对结肠癌细胞系HT-29的体外诱导凋亡效应
- Author:
Lina WU
;
Zhixin LI
;
Tian LU
;
Teng MA
;
Xiaoyan QIU
- Publication Type:Journal Article
- Keywords:
Genes,suppressor;
Hyperthermia,induced;
Cell proliferation;
Apoptosis
- From:
Journal of Peking University(Health Sciences)
2003;0(05):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the suppression effect and apoptosis of the colon cell line HT-29 treated with adenovirus-expressed human hyperplasia suppressor gene(hHSG)and hyperthermia.Methods:Replication-defective recombinant adenovirus containing hHSG was constructed,and then infected into HT-29 cell line to detect its overexpression by western blot.The synergic effect with hyperthermia was observed,cell survival was assessed using Vi-CELL TMXR Cell Viability Analyzer,and cell apoptosis and cell cycle distribution were determined by flow cytometry.Results:After the cells were treated with 100 MOI(multiplicity of infection)Ad5-hHSG and heated at 42 ℃ for 1 h,the number of viable cells decreased sharply,compared with single treatment with Ad5-hHSG or 42 ℃ for 1 h(P
