The Antitumor Effects in vitro and Impact on Colon Cancer in Rats of Endostatin Transfected Bifidobacterium Oral Powder Preparation
- VernacularTitle:血管内皮抑素转基因制剂的体外抗肿瘤作用及对大鼠大肠癌的影响
- Author:
Chengnong GUAN
;
Haiqing LUO
;
Xianghui LIAO
;
Xinming CHEN
- Publication Type:Journal Article
- Keywords:
Endostatin transfected bifidobacterium oral powder preparation(ETB-2);
Colon cancer;
1,2-dimethylhydrazine(DMH)
- From:
Journal of Medical Research
2006;0(10):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the antitumor effects in vitro and impact on colon cancer in rats of endostatin transfected bifidobacterium oral powder preparation(ETB-2).Methods Growth inhibitory effect of the cecropins on normal human gastric epithelial cell line(GES-1) and human colon adenocarcinoma cell line(LS-174T) was observed using a microculturetetrazolium(MTT) colorimetric methods.Male Wistar rats were divided into 4 groups.All treatments were completed in a course of 18 weeks and the experiment was finished at week 33.Results The cecropins showed selective cytotoxic activity against the human colon adenocarcinoma cell line.There was a significant lower in incidence of colon tumors in rats(70%vs100%,P0.05).Conclusion ETB-2 has significant preventive effect on colon cancer induced by DMH in rats.
