The Analysis of Mouse Blood Serum and Colture of Primary Hepatocytes Induced by Phenobarbital Sodiu
- VernacularTitle:苯巴比妥钠诱导小鼠后血清与原代肝细胞培养物的生物学分析
- Author:
Tonghe WU
;
Nanqing CHEN
;
Yongqing HUANG
;
Aijing CHEN
;
Yanfen SHI
- Publication Type:Journal Article
- Keywords:
Phenobarbital sodium(PBS);
Preinduce;
Adult mice;
Total protein
- From:
Journal of Medical Research
2006;0(10):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the biological characteritics of adult animal hepatocytes induced by phenobarbital sodium(PBS) in vivo,and to study the potential value of biological artificial liver of effective hepatocytes.Methods 12 adult male mice,are yandomly divided in to preinducing group and controll group.The preinducing group are intraperitoneally injected PBS per day,45mg/kg for 7 times in total;the controll ware injected NS.after that,we detected the blood serum TP,BUN,CHOL,HDLC.And the same amount of isolative hepatocytes was developed after being developed 48h;MTT was used to investigate the proliferation of hepatocytes after being developed 24h;chromosome was investigated to observe the cell division;and the survival deadline and morphology was also investigated.Results The TP had remarkable difference between the two groups(t=2.678,P
