STAT3 participates in the injury of alveolar type Ⅱ epithelial cells during severe acute pancreatitis:an in vitro study
- VernacularTitle:STAT3对大鼠重症急性胰腺炎血清体外作用AT-Ⅱ SP-C的影响
- Author:
Dunjing ZHONG
;
Hong GUO
;
Jia HAO
;
Xiaoyan ZHAO
- Publication Type:Journal Article
- Keywords:
STAT3;
severe acute pancreatitis;
alveolar type Ⅱ epithelial cells;
surfactant protein C
- From:Journal of Third Military Medical University
2003;0(19):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the role of signal transducer and activator of transcription3 (Stat3) in alveolar type Ⅱ epithelial cells (AT-Ⅱ) treated with the serum from rat model of severe acute pancreatitis. Methods AT-Ⅱ cells of primary culture were treated with serum from rat model of severe acute pancreatitis (SAP group),or SAP serum+AG490 (JAK inhibitor),while the normal cell control was set. AT-Ⅱ cells after treatment were collected to determine activation of Stat3 by EMSA,Stat3 mRNA expression by RT-PCR,and surfactant protein C (SP-C) level in AT-Ⅱ cells by flow cytometry. Results Stat3 protein and mRNA levels were enhanced in SAP group (P
