Construction of BaF3-P210 cell line stably expressing BCR/ABL protein and its biological activity
- VernacularTitle:稳定表达BCR/ABL的小鼠BaF3-P210细胞株的建立及其生物学特性的研究
- Author:
Meng SHI
;
Wenli FENG
;
Wenping ZHANG
;
Xiaozhong WANG
;
Shifeng HUANG
;
Jianming ZENG
;
Jianping WEN
;
Kun TAO
;
Xinmin CHEN
;
Weixi CAO
;
Zonggan HUANG
- Publication Type:Journal Article
- Keywords:
chronic myeloid leukemia;
bcr/abl fusion gene;
retroviral vector;
BaF3 cell line
- From:Journal of Third Military Medical University
2003;0(19):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To construct the transformed mouse BaF3-P210 cell line stably expressing BCR/ABL and to initially investigate the influence of BCR/ABL on the cell biological characteristics of BaF3 cell line. Methods The retroviral vector with bcr/abl gene was transfected into the packaging cell line. The BaF3 cells were infected with the collected viral supernatant. The transgenic BaF3-P210 cell line stably expressing BCR/ABL were screened and subcloned. The integration of the bcr/abl gene in the genome of the target cell was determined by PCR and DNA sequencing,trypan blue staining assay,flow cytometry and MTT assay. Results The bcr/abl gene was integrated into the BaF3 cell genome; RT-PCR and Western blot verified the stable expression of the bcr/abl gene and protein in the screened subclone cell line BaF3-P210. Compared with the control group,the cell proliferation rate was promoted (P
