Study on histochemical localization of phenol oxidase in Oncomelania hupensis
- VernacularTitle:湖北钉螺酚氧化酶组织化学定位进一步研究
- Author:
Lei ZHAO
;
Shulin ZHOU
;
Xiaoniu TANG
- Publication Type:Journal Article
- Keywords:
Oncomelania hupensis;
Phenol oxidase;
Enzyme histochemistry
- From:
Chinese Journal of Schistosomiasis Control
1991;0(05):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the histological distribution of phenol oxidase (PO) in body of Oncomelania hupensis. Methods Oncomelania hupensis snails collected from the riverside of Wuhu section of the Yangtze River were anatomized and the unimpaired software organs were sliced in frozen condition to get the continued sections, then the sections were incubated in pH 6.8 phosphate buffer containing 25 mmol/L catechol at 37 ℃ and investigated systematically for histological distribution of PO in snail bodies. Results The light microscope showed that there were black grannles of PO (histochemical reaction) in the liver, ctenidium, head-foot, tentacle and mantle collar of snail body. Conclusion PO exists in Oncomelania hupensis and locates in the liver, mantle collar, head-foot, tentacle and ctenidium.
