Toxic effects of methylmercury, arsanilic acid and danofloxacin on the differentiation of mouse embryonic stem cells into neural cells.
- Author:
Seok Jin KANG
1
;
Sang Hee JEONG
;
Eun Joo KIM
;
Young Il PARK
;
Sung Won PARK
;
Hyo Sook SHIN
;
Seong Wan SON
;
Hwan Goo KANG
Author Information
- Publication Type:Original Article ; Research Support, Non-U.S. Gov't
- Keywords: arsanilic acid; danofloxacin; embryonic stem cell test; methylmercury; neural cell
- MeSH: Acetylcholinesterase/metabolism; Animals; Arsanilic Acid/*toxicity; Cell Differentiation/*drug effects; Embryonic Stem Cells/cytology/*drug effects; Environmental Pollutants/*toxicity; Fluorescent Antibody Technique; Fluoroquinolones/*toxicity; Gene Expression Regulation/drug effects; Methylmercury Compounds/*toxicity; Mice; Nerve Tissue Proteins/metabolism; Neurons/cytology/*drug effects; Tetrazolium Salts/metabolism; Thiazoles/metabolism
- From:Journal of Veterinary Science 2014;15(1):61-71
- CountryRepublic of Korea
- Language:English
- Abstract: This study was performed to assess the neurotoxic effects of methylmercury, arsanilic acid and danofloxacin by quantification of neural-specific proteins in vitro. Quantitation of the protein markers during 14 days of differentiation indicated that the mouse ESCs were completely differentiated into neural cells by Day 8. The cells were treated with non-cytotoxic concentrations of three chemicals during differentiation. Low levels of exposure to methylmercury decreased the expression of GABAA-R and Nestin during the differentiating stage, and Nestin during the differentiated stage. In contrast, GFAP, Tuj1, and MAP2 expression was affected only by relatively high doses during both stages. Arsanilic acid affected the levels of GABA(A)-R and GFAP during the differentiated stage while the changes of Nestin and Tuj1 were greater during the differentiating stage. For the neural markers (except Nestin) expressed during both stages, danofloxacin affected protein levels at lower concentrations in the differentiated stage than the differentiating stage. Acetylcholinesterase activity was inhibited by relatively low concentrations of methylmercury and arsanilic acid during the differentiating stage while this activity was inhibited only by more than 40 microM of danofloxacin in the differentiated stage. Our results provide useful information about the different toxicities of chemicals and the impact on neural development.
