Determination of baicalin in Zhisou Kechuanning syrup by HPLC
- VernacularTitle:HPLC法测定止嗽咳喘宁糖浆中黄芩苷的含量
- Author:
Xiarong SHI
;
Yuanjun TANG
- Publication Type:Journal Article
- Keywords:
Baicalin;
Zhisou Kechuanning Syrup;
Content determination;
HPLC
- From:
China Journal of Traditional Chinese Medicine and Pharmacy
2005;0(10):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To determine the content of baicalin in Zhisou Kechuanning syrup.Methods:.HPLC was used and di erent derivatization reaction conditions were investigated with the optimal conditions found to be the following:the chromatography column was Waters symmetry C 18(5?m;4.6?150mm),the mobile phase(solvent) was a mixture of methanol-0.1% Phosphoric acid(45:55)with a ow rate of 0.8 ml/min,and the detection wavelength was 280nm.Results:The linear range of Bergenin was 0.41-8.13?g with correlation factor r=0.9997,the average recovery was 98.36% and RSD=1.08%.Conclusion:This method is simple,reproducible,and can be used for quantitative determination of baicalin in Zhisou Kechuanning Syrup.
