Determination of Bergenin in Dicha Kechuan oral liquid by HPLC
- VernacularTitle:HPLC法测定地茶咳喘露中岩白菜素的含量
- Author:
Dejian WEN
;
Hui ZHAO
;
Shengyan XI
;
Jinghua ZHAO
- Publication Type:Journal Article
- Keywords:
HPLC;
Dicha Kechuan oral liquid;
Bergenin;
Content determination
- From:
China Journal of Traditional Chinese Medicine and Pharmacy
2005;0(10):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To establish a determination method of Bergenin content in Dicha Kechuan oral liquid by HPLC.Methods:The separation was performed on SHIMADZU VP-ODS C18 column(150mm?4.6mm,5?m),the mobile phase was carbinol-water(20:80) with a flow rate of 1.0ml/min.The column temperature was room temperature and the detection wavelength was 275nm.Results:The linear range of Bergenin was 0.2575-5.150?g,the regression equation of working curve was Y=812702.2X-80626.3,r=0.9998,the average recovery was 101.4% and RSD 1.67%(n=5).Conclusion:This method is simple,reproducible,and can be used for quality control of Dicha Kechuan Oral Liquid and the semifinished product.
