Protective effects of Caspase-1 inhibitor on lung injury induced by experimental severe acute pancreatitis
- VernacularTitle:Caspase-1抑制剂对实验性重症急性胰腺炎肺损伤的保护作用
- Author:
Xiaohua ZHANG
;
Hongzan JI
;
Miaofang YANG
- Publication Type:Journal Article
- Keywords:
severe acute pancreatitis;
respiratory distress syndrome,adult;
Caspase-1;
interleukin-1?;
interleukin-18
- From:
Medical Journal of Chinese People's Liberation Army
2001;0(10):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To evaluate the protective effect of Caspase-1 inhibitor on acute lung injury(ALI) induced by experimental severe acute pancreatitis(SAP).Methods Forty-two SD rats were randomly assigned into 3 groups: healthy controls group(HC,n=6),SAP+normal saline group(SAP-S,n=18) and SAP+ICE inhibitor group(SAP-ICE-I,n=18).SAP was reproduced by retrograde injection of 5% sodium taurocholate into the pancreaticobiliary duct in SD rats.In animals in HC group same surgical procedure and duct cannulation were performed but without introduction of sodium taurocholate.In SAP-S group,rats received the first intraperitoneal injection of isotonic saline 2h after induction of acute pancreatitis,and the injection of saline was repeated at the 12th hour after induction.In SAP-ICE-I group,rats were given ICE inhibitor intraperitoneally 2h after induction of pancreatitis.Surviving rats were sacrificed at designated time points,and blood and all organs were obtained for subsequent analysis.Serum IL-1? was measured by ELISA.Intrapulmonary expressions of Caspase-1,IL-1? and IL-18 mRNA were detected by semi-quantitative RT-PCR.The ratios of wet/dry weight and histopathological changes in lung were also evaluated.Results The serum IL-1? levels in SAP-S group were 276.77?44.92pg/ml at 6h,308.99?34.95pg/ml at 12h and 311.60?46.51pg/ml at 18h after induction of SAP,respectively,which were increased significantly compared with that in HC group(P0.05).The wet/dry weight ratio of lung in SAP-S group was increased significantly compared with that in HC group(P
