Positive effects of porcine IL-2 and IL-4 on virus-specific immune responses induced by the porcine reproductive and respiratory syndrome virus (PRRSV) ORF5 DNA vaccine in swine.
- Author:
Deyuan TANG
1
;
Jian LIU
;
Chunyan LI
;
Hua ZHANG
;
Ping MA
;
Xianfeng LUO
;
Zhiyong ZENG
;
Nining HONG
;
Xia LIU
;
Bin WANG
;
Feng WANG
;
Zhenlei GAN
;
Fei HAO
Author Information
- Publication Type:Original Article ; Research Support, Non-U.S. Gov't
- Keywords: IL-2; IL-4; immunogenicity; ORF5 DNA vaccine; porcine reproductive and respiratory syndrome virus
- MeSH: Animals; Cell Line; Escherichia coli/genetics; Haplorhini; Immunity, Cellular; Interleukin-2/genetics/*metabolism; Interleukin-4/genetics/*metabolism; Neutralization Tests/veterinary; Plasmids; Porcine Reproductive and Respiratory Syndrome/*prevention & control; Porcine respiratory and reproductive syndrome virus/*immunology; Recombinant Proteins/genetics/metabolism; Swine; Vaccines, DNA/immunology; Viral Envelope Proteins/*genetics/metabolism; Viral Vaccines/*immunology
- From:Journal of Veterinary Science 2014;15(1):99-109
- CountryRepublic of Korea
- Language:English
- Abstract: The purpose of this study was to investigate the effects of porcine interleukin (IL)-2 and IL-4 genes on enhancing the immunogenicity of a porcine reproductive and respiratory syndrome virus ORF5 DNA vaccine in piglets. Eukaryotic expression plasmids pcDNA-ORF5, pcDNA-IL-2, and pcDNA-IL-4 were constructed and then expressed in Marc-145 cells. The effects of these genes were detected using an indirect immunofluorescent assay and reverse transcription polymerase chain reaction (RT-PCR). Characteristic fluorescence was observed at different times after pcDNA-ORF5 was expressed in the Marc-145 cells, and PCR products corresponding to ORF5, IL-2, and IL-4 genes were detected at 48 h. Based on these data, healthy piglets were injected intramuscularly with different combinations of the purified plasmids: pcDNA-ORF5 alone, pcDNA-ORF5 + pcDNA-IL-2, pcDNA-ORF5 + pcDNA-IL-4, and pcDNA-ORF5 + pcDNAIL-4 + pcDNA-IL-2. The ensuing humoral immune responses, percentages of CD4+ and CD8+ T lymphocytes, proliferation indices, and interferon-gamma expression were analyzed. Results revealed that the piglets co-immunized with pcDNA-ORF5 + pcDNA-IL-4 + pcDNA-IL-2 plasmids developed significantly higher antibody titers and neutralizing antibody levels, had significantly increased levels of specific T lymphocyte proliferation, elevated percentages of CD4+ and CD8+ T lymphocytes, and significantly higher IFN-gamma production than the other inoculated pigs (p < 0.05).
