Expression of striatin gene and protein declined in brain of drug-refractory epilepsy
- VernacularTitle:耐药性颞叶癫痫脑组织中隆纹菌素的表达及临床意义
- Author:
Lifeng GUANG
;
Xuefeng WANG
;
Tao LIN
- Publication Type:Journal Article
- Keywords:
STRN protein,human;
epilepsy;
receptors,estrogen;
temporal lobe;
nitric oxide
- From:
Medical Journal of Chinese People's Liberation Army
2001;0(10):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the expression of striatin(STRN) protein in brain tissue of paitents with drug-refractory epilepsy,and analyze the clinical significance of the expression.Methods Immunohistochemistry,immunofluorescence,cDNA array and Western blot were used to measure the levels of STRN cDNA and the protein in surgically removed temporal lobe tissues of patients with refractory epilepsy.Samples were retrieved from the brain bank of the authors' department(n=42),and the results were compared with that of the normal controls(n=12).Results STRN cDNA of STRN was abnormally expressed in the temporal cortex of the patients with epilepsy,the ratio of Cy5/Cy3 of STRN was 0.384.Moreover,the expression of STRN was significantly lower in neurons and glial cells of all epilepsy specimens.OD value in epileptic tissues determined by Immunohistochemical method was 0.310 8?0.108 7,which was significantly lower than that of control(0.421 0?0.120 2,P
