Expression of HIF-1? after focal cerebral ischemia/reperfusion in rats
- VernacularTitle:局灶性脑缺血再灌注后大鼠脑组织HIF-1?表达的观察
- Author:
Kaijian LUO
;
Tao TAO
;
Jin DENG
;
Lan YE
- Publication Type:Journal Article
- Keywords:
focal cerebral ischemia;
hypoxia inducible factor-1?
- From:Journal of Third Military Medical University
2003;0(18):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the expression of hypoxia inducible factor-1 alpha(HIF-1?) and HIF-1? mRNA after focal cerebral ischemia reperfusion in rats.Methods Adult male SD rats were divided into six groups: sham operation group,5 groups of ischemia/reperfusion(the rats undergoing 2-hour ischemia and then 6-hour,1-day,2-day,3-day and 4-day reperfusion),with 6 rats in each group.Middle cerebral artery occlusion(MCAO) was set up in male SD rats according to Longa' method.The cerebral tissues were analyzed with immunohistochemical methods.The level of HIF-1? protein by immunohistochemical methods and the level of HIF-1? mRNA by in situ hybridization and RT-PCR were detected in the brain tissues.Results HIF-1? protein expression started at 6 h after reperfusion,increased remarkably on day 1,reached the peak on day 3,then declined markedly on day 4.HIF-1? mRNA increased at 6 h after reperfusion,peaked on day 3,then declined on day 4.HIF-1? expression was mainly in the periphery of infarcted area at each time point.Conclusion High expression of HIF-1? during focal cerebral ischemia/reperfusion in the rat brain tissues,suggests that HIF-1? has neuroprotective effect against cerebral ischemic injury.
