Growth inhibition effects of artesunate on human cervical cancer cell line HeLa
- VernacularTitle:青蒿琥酯对人子宫颈癌细胞株HeLa凋亡与增殖的影响
- Author:
Minghua WANG
;
Junsheng ZHENG
;
Yunpeng LUO
;
Can MI
- Publication Type:Journal Article
- Keywords:
artesunate;
HeLa cell line;
reactive oxygen species;
mitochondrial member potential;
apoptosis
- From:Journal of Third Military Medical University
2003;0(18):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effects of artesunate on human cervical cancer cell line HeLa.Methods The inhibitory effect on cell proliferation was assessed by MTT assay.Transmission electron microscopy and fluorescent staining were applied to demonstrate the presence of apoptosis.Cell cycle,reactive oxygen species(ROS) levels and mitochondrial membrane potential(??m) were examined by flow cytometry,respectively.Expression of caspase-3 was detected by immunohistochemical methods.Results Growth inhibition of ART on HeLa cells was time-and dose-dependent.Apoptotic feature was observed by transmission electron microscopy and fluorescent staining.The blocking of the cell cycle was in S-phase.The expression of caspase-3 in cytoplasm was positive.The generation of ROS increased obviously(P
