Establishment of breast cancer cell sublines with different potential of metastasis and their biological characteristics
- VernacularTitle:不同转移潜能乳腺癌细胞亚系的建立与生物学特性的分析
- Author:
Tao ZHANG
;
Weixue TANG
;
Hui CAI
;
Shaolin LI
- Publication Type:Journal Article
- Keywords:
breast cancer;
neoplasm metastasis;
cell subclone;
biological characteristics
- From:Journal of Third Military Medical University
2003;0(18):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the validity of selecting subclones of human breast cancer cells with high-and low-metastatic potentiality,based on their different ability of penetrating the matrigel in vitro,and analyze their biological characteristics.Methods Continuous in vitro invasion assays in matrigel were applied to screen two subclones of human breast cancer cell line MDA-MB-435s,characterized by high-and low-metastatic potentiality.Cell growth curve assay,invasion assay,adhesion assay and angiopoiesis-promoting assay were conducted to explore the different biological properties of these two subclone cells.Results Two subclones of MDA-MB-435s,of high-and low-metastatic potentiality respectively,were obtained through continuous in vitro invasion assay,manifesting significant difference in growth rate,invasion ability,adhesion ability and angiopoiesis-promoting ability.Conclusion It is feasible to obtain subclones of human breast cancer cells of high-and low-metastatic potentiality by utilizing their difference in penetrating the matrigel in vitro.
