Regulation of matrix metalloproteinase-9 protein expression by 1alpha,25-(OH)2D3 during osteoclast differentiation.
10.4142/jvs.2014.15.1.133
- Author:
Jian Hong GU
1
;
Xi Shuai TONG
;
Guo Hong CHEN
;
Xue Zhong LIU
;
Jian Chun BIAN
;
Yan YUAN
;
Zong Ping LIU
Author Information
1. College of Veterinary Medicine Yangzhou University, Yangzhou 225009, China. bianjianchun@sina.com
- Publication Type:Original Article ; Research Support, Non-U.S. Gov't
- Keywords:
1alpha,25-(OH)2D3;
bone lacunar resorption;
MMP-9;
osteoclast;
TRAP
- MeSH:
Acid Phosphatase/metabolism;
Animals;
Blotting, Western;
Calcitriol/*pharmacology;
Calcium Channel Agonists/pharmacology;
*Cell Differentiation;
Cell Line;
Cell Proliferation;
Gene Expression Regulation, Enzymologic/*drug effects;
Isoenzymes/metabolism;
Matrix Metalloproteinase 9/*genetics/metabolism;
Mice;
Osteoclasts/*cytology/*enzymology;
Tetrazolium Salts;
Thiazoles
- From:Journal of Veterinary Science
2014;15(1):133-140
- CountryRepublic of Korea
- Language:English
-
Abstract:
To investigate 1alpha,25-(OH)2D3 regulation of matrix metalloproteinase-9 (MMP-9) protein expression during osteoclast formation and differentiation, receptor activator of nuclear factor kappaB ligand (RANKL) and macrophage colony-stimulating factor (M-CSF) were administered to induce the differentiation of RAW264.7 cells into osteoclasts. The cells were incubated with different concentrations of 1alpha,25-(OH)2D3 during culturing, and cell proliferation was measured using the methylthiazol tetrazolium method. Osteoclast formation was confirmed using tartrate-resistant acid phosphatase (TRAP) staining and assessing bone lacunar resorption. MMP-9 protein expression levels were measured with Western blotting. We showed that 1alpha,25-(OH)2D3 inhibited RAW264.7 cell proliferation induced by RANKL and M-CSF, increased the numbers of TRAP-positive osteoclasts and their nuclei, enhanced osteoclast bone resorption, and promoted MMP-9 protein expression in a concentration-dependent manner. These findings indicate that 1alpha,25-(OH)2D3 administered at a physiological relevant concentration promoted osteoclast formation and could regulate osteoclast bone metabolism by increasing MMP-9 protein expression during osteoclast differentiation.
