Effects of smoking and glonoine on intracellular Ca~(2+) in vascular smooth muscle cell in rabbits with atherosclerosis
- VernacularTitle:吸烟、硝酸甘油对动脉粥样硬化家兔血管平滑肌细胞钙浓度的影响
- Author:
Shanting LIU
;
Lizan WANG
;
Jiang YU
;
Jianmei LI
;
Qin XIN
;
You WANG
;
Fanhe ZHU
;
Jun LI
;
Ruxia QI
- Publication Type:Journal Article
- Keywords:
atherosclerosis;
smoking;
glonoine;
vascular smooth muscle cell;
intracellular free calcium concentration;
flow cytometry;
laser scanning confocal microscopy
- From:
Chinese Pharmacological Bulletin
2003;0(09):-
- CountryChina
- Language:Chinese
-
Abstract:
Aim To study the effects of smoking and glonoine on intracellular free Ca2+ concentration([Ca2+]i) in vascular smooth muscle cell in rabbits with atherosclerosis,and to explore the effects of smoking on atherosclerosis and biologic action of glonoine.Methods An atherosclerosis in rabbits was produced.The vascular smooth muscle cells were isolated.The cells were loaded by Fluo-3/AM.[Ca2+]i in vascular smooth muscle cell was measured by flow cytometer(FCM).The spatial distribution and the dynamic changes of [Ca2+]i in single vascular smooth muscle cells were determined by laser scanning confocal microscopy(LSCM).Results Atherosclerosis plaques in arteriae aorta were observed and the degrees were different in various groups.[Ca2+]i in vascular smooth muscle cells in rabbits with atherosclerosis markedly increased[(48.45?5.31) vs that in saline control(38.09?2.57),P
