Expression of mGluR1 in hippocampus of spontaneously epileptic rats
- VernacularTitle:mGluR1在自发性癫痫大鼠海马中的表达
- Author:
Qinghua WANG
;
Dan YANG
;
Jiqun CAI
;
Feng SUN
- Publication Type:Journal Article
- Keywords:
mGluR1;
RT-PCR;
immunohistochemistry;
Western blot;
spontaneously epileptic rats
- From:
Chinese Pharmacological Bulletin
2003;0(09):-
- CountryChina
- Language:Chinese
-
Abstract:
Aim To investigate the expression of mGluR1 in hippocampus of spontaneously epileptic rats(SER) by control study.Methods Total RNA was extracted from hippocampus of SER and Wistar rats,and mGluR1 mRNA expression was detected by RT-PCR;the expression of mGluR1 protein was detected by immunohistochemistry and Western blot.Results mGluR1 mRNA of SER was lower than that of Wistar rats in hippocampus(P0.05) were found.In positive cells,mGluR1 proteins were enhanced.By Western blot,total mGluR1 protein was down-regulated in SER hippocampus(P
