Effects of gavage with lactococcus lactis recombinant heme oxygenase-1 gene on intestinal barrier in rats with endotoxemia
- VernacularTitle:血红素加氧酶-1重组乳酸乳球菌灌胃对内毒素血症大鼠肠道的保护作用
- Author:
Yun LI
;
Qiaomei ZHOU
;
Yinming ZENG
;
Qingfeng PANG
- Publication Type:Journal Article
- Keywords:
heme oxygenase-1;
endotoxemia;
L.Lactis;
glutamine;
inflammation of lower intestine
- From:
Chinese Pharmacological Bulletin
2003;0(09):-
- CountryChina
- Language:Chinese
-
Abstract:
Aim To evaluate the effects of gavage with lactococcus lactis(L.Lactis) recombinant heme oxyge-nase-1(HO-1) gene on alleviation of intestinal inflammation and protection of the intestinal mucosa in rats with endotoxemia.Methods A model of rats with endotoxemia was produced in 24 healthy SD male rats.The rats were randomly divided into the L.Lactis recombinant HO-1(LL-HO-1)gene group(HO-1 group,n=8),L.Lactis group(LL group,n=8) and glutamine group(Glu group,n=8).The rats received 1 ml(5?1010 CFU?ml-1)?d-1LL-HO-1,1 ml(5?1010 CFU?ml-1)?d-1 L.Lactis or 1 g?kg-1?d-1 glutamine for four days before LPS intraperitoneal injecion.Twenty hours after LPS injection,the full-thickness distal ileum was harvested.The mortality,myeloperoxidase(MPO) activity,the pathological changes,the contents of HO-1,tumor necrosis factor-?(TNF-?) and interleukin-10(IL-10) in the intestine were determined.Results When the results of HO-1 group was compared with those of LL group,the survival rate was significantly elevated in the former(P
