Troglitazone inhibits proliferation of GH3 cell in vitro
- VernacularTitle:曲格列酮体外抑制大鼠垂体腺瘤GH3细胞增殖
- Author:
Fuyong CHEN
;
Shousen WANG
;
Shuiliang WANG
;
Rumi WANG
- Publication Type:Journal Article
- Keywords:
pituitary adenoma;
peroxisome proliferator-activated receptor ?,thiazolidinedione compounds;
troglitazone;
GH3 cell;
cell proliferation
- From:
Academic Journal of Second Military Medical University
2001;0(09):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To study the anti-proliferation effects of thiazolidinedione compounds-troglitazone, which is a high affinity ligand of PPAR-? , on rat pituitary adenoma GH3 cell line and explore the related mechanisms.Methods: GH3 cells were separately treated with troglitazone (10-7,10-6 and 10-5 mol/L), dimethyl sulfoxide (DMSO) (DMSO control group) and phenol red- and serum-free F-12 medium (blank group). MTT was used to examine the cell growth in each group and FACS was used to detect the distribution of cell cycle. Semi-quantitative RT-PCR method was utilized to determine the expression of CyclinD1 mRNA. ANOVA was used for statistical analysis.Results:The 72 h treatment with troglitazone inhibited GH3 cell proliferation in a dose-Dependent manner. The treatment also induced cell cycle arrest in G1/S phase and significantly decreased the expression of CyclinD1 mRNA as compared to the other 2 groups (P
