Rhesus Model-based Immunological Insights into Endometriosis.
- VernacularTitle:猕猴子宫内膜异位症模型免疫系统的研究
- Author:
Lili ZONG
;
Longxia WANG
;
Yali LI
- Publication Type:Journal Article
- Keywords:
Rhesus;
Endometriosis;
Immunity;
CA-125
- From:
Journal of Medical Research
2006;0(09):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To Investigate the dynamics of the parameters in the immune system,including T cell,NK cell sub-populations and cytokines,on the experimental endometriosis model in rhesus,in order to study the immunity-related profile in endometriosis.Materials Six female healthy rhesuses with regular menstrual cycle were selected,among them,five for experiment and one as control.During day 8-15 of menstruation,or day 3-5 after the midcycle estrogen peak,the endometrium and the greater omentum were implanted into the extra-uterine pelvic cavity in the experimental group and the control group,respectively.Samples of sera were collected at the time of 8th and 16th day of every menstruation after the implantation.An exploratory laparotomy or laparoscopy was undertaken during the period from day 8 to day 15 of the menstruation in the 2nd month and the 4th month after the implantation for observation of the occurrence of endometriosis.Methods Flow cytometer(FCM),radioimmuno-assay(RIA),electrochemiluminescence(ECL) and enzyme-linked immunosorbent assay(ELISA) were used to detect the dynamics of the expression levels of CD3,CD4,CD8,CD16 and activity of NK cells,the dynamics of the IgG、IgM,of cytokines,including TNF-a、IL-6、IL-8、CA125、HGF and ICAM-1 in peripheral blood of rhesus pre-operation and during the three months of post operation.Results Cellular immune function declined in the rhesus endometriosis model,showing from the changes of the CD3、CD4、NK(CD16) ratios,CD4/CD8 ratio and decrease of the NK cell activity;For the humoral immunity,no significant changes occurred in the levels of IgG、IgM,with little decline.5 During the 4-month period post-operation,the levels of CA125 rised gradually,ICAM-1 and HGF increased significantly,IL-8 increased slightly,TNF and IL-6 increased markedly in rhesuses that formed endometriosis.Conclusion This experiment shows what we observed in human patients for the immunological system changes could also be reproduced in the rhesus model.Therefore such a model can be used in the future to test the interference of the immune system to tackle his medical problem.Finally we found the oncogenis marker CA125 shows a gradual increase during the disease course therefore it can be used for prognosis of the disease.
