Development of a multiplex PCR assay to detect Edwardsiella tarda, Streptococcus parauberis, and Streptococcus iniae in olive flounder (Paralichthys olivaceus).
10.4142/jvs.2014.15.1.163
- Author:
Seong Bin PARK
1
;
Kyoung KWON
;
In Seok CHA
;
Ho Bin JANG
;
Seong Won NHO
;
Fernand F FAGUTAO
;
Young Kyu KIM
;
Jong Earn YU
;
Tae Sung JUNG
Author Information
1. Aquatic Biotechnology Center, College of Veterinary Medicine, Gyeongsang National University, Jinju 660-701, Korea. jungts@gnu.ac.kr
- Publication Type:Brief Communication ; Research Support, Non-U.S. Gov't
- Keywords:
Edwardsiella tarda;
multiplex PCR;
olive flounder;
Streptococcus iniae;
Streptococcus parauberis
- MeSH:
Animals;
Edwardsiella tarda/genetics/*isolation & purification;
Enterobacteriaceae Infections/diagnosis/microbiology/*veterinary;
Fish Diseases/*diagnosis/microbiology;
Fisheries/*methods;
*Flatfishes;
Multiplex Polymerase Chain Reaction/economics/*veterinary;
Sensitivity and Specificity;
Streptococcal Infections/diagnosis/microbiology/*veterinary;
Streptococcus/genetics/*isolation & purification
- From:Journal of Veterinary Science
2014;15(1):163-166
- CountryRepublic of Korea
- Language:English
-
Abstract:
A multiplex PCR protocol was established to simultaneously detect major bacterial pathogens in olive flounder (Paralichthys olivaceus) including Edwardsiella (E.) tarda, Streptococcus (S.) parauberis, and S. iniae. The PCR assay was able to detect 0.01 ng of E. tarda, 0.1 ng of S. parauberis, and 1 ng of S. iniae genomic DNA. Furthermore, this technique was found to have high specificity when tested with related bacterial species. This method represents a cheaper, faster, and reliable alternative for identifying major bacterial pathogens in olive flounder, the most important farmed fish in Korea.
