Antisense oligonucleotide targeting livin induces apoptosis of human renal carcinoma cell 786-O in vitro and its mechanism
- VernacularTitle:Livin反义寡核苷酸诱导人肾癌细胞凋亡及其机制的研究
- Author:
Chuan LIU
;
Xiaohou WU
;
Weili ZHANG
;
Wei TANG
;
Zhikang YIN
;
Yunfeng HE
;
Hu DU
- Publication Type:Journal Article
- Keywords:
livin;
oligonucleotides, antisense;
apoptosis;
renal tumor
- From:Journal of Third Military Medical University
2003;0(17):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of antisense oligonucleotides (ASODN) targeting livin on the inhibition of livin mRNA and protein expression and the apoptosis of human renal carcinoma cell line 786-O cells. Methods Specific phosphorothioate antisense oligodeoxynucleotides targeting livin were synthesized and then transfected into 786-O cells. The expressions of livin mRNA were detected by RT-PCR. Expression and location of livin protein were observed by confocal laser scanning microscope (CLSM). Apoptosis rate of 786-O cells was investigated by flow cytometer. The activity of Caspase-3 was detected by colorimetric assay. Results After the transfection of ASODN, the expression of livin mRNA was decreased (P
