Superparamagnetic iron oxide labeled mesenchymal stem cells and their magnetic resonance imaging in vitro
- VernacularTitle:SPIO标记大鼠骨髓基质干细胞及体外磁共振成像研究
- Author:
Hua YANG
;
Xiaoming ZHANG
;
Yang SHAO
;
Hong JIANG
;
Zhaohua ZHAI
- Publication Type:Journal Article
- Keywords:
superparamagnetic iron oxides;
poly-L-lysine;
bone mesenchymal stem cells;
magnetic resonance image
- From:Journal of Third Military Medical University
2003;0(17):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the optimal situation of labeling bone mesenchymal stem cells (BMSCs) with superparamagnetic iron oxides (SPIO) mediated by poly-L-lysine (PLL), and determine the most optimal protocol of magnetic resonance imaging according to the patterns of MR in vitro. Methods BMSCs were isolated from white rat and purified, incubated with SPIO-PLL complexes at the range of concentrations (0, 4.2, 8.4, 21, 42, 84 ?g Fe per ml medium). The labeling ratio and distribution of SPIO particles in BMSCs, and the morphological evidence of abnormal visualization were evaluated by Prussian blue staining, fluorescent microscope and electron microscopy. MTT growth curves and magnetic resonance imagings were obtained at the range of concentrations. Trypan blue exclusion test was performed to elevate the viability of BMSCs labeled with PLL at the range of concentrations (0, 0.05, 0.25, 0.5, 1.0, 5.0 ?g PLL per ml medium). Results The cellular labeling ratio was strongly correlated to the concentrations of SPIO (P
