Effects of infusion of bone marrow mesenchymal stem cells on proliferation and apoptosis in gentamicin-induced acute renal failure in rat
- VernacularTitle:骨髓间质干细胞输注对庆大霉素致急性肾小管损伤细胞增殖和凋亡的影响
- Author:
Hongbao LIU
;
Feng ZHAO
;
Peng ZHANG
- Publication Type:Journal Article
- Keywords:
reperfusion injury;
kidney;
mesenchymal stem cells;
apoptosis;
genes
- From:
Medical Journal of Chinese People's Liberation Army
2001;0(09):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effects of mesenchymal stem cells(MSCs) on the recovery of gentamicin-induced acute renal failure(ARF),and explore its possible mechanism.Methods MSCs were isolated from a male SD rat and labeled with DAPI before injection.ARF rat model was reproduced by hypodermic injection of gentamicin.At the time that gentamicin was injected,MSCs were injected through tail vein.Thirty SD rats were randomly divided into three groups(10 each): MSCs injection group(group M),DMEM-F12 infusion group(group D) and control group(group C).Rats were sacrificed on day 7 after gentamicin injection.The levels of serum Scr and BUN were determined,and the renal tissue was sampled for pathological observation.Cell apoptotic index(AI) was calculated after terminal deoxynucleotidyl transferase mediated d-UTP nick end labeling(TUNEL).The expressions of Bcl-2,Fas and FasL proteins and PCNA were determined by immunohistochemical technique.The distribution of DAPI positive MSCs in kidney of recipient SD rats was examined by immunohistochemistry.Results HSK score(5.5?0.8 vs 10.2?0.8,P
