Influence of optic nerve transection on the expression of Nogo A/B and NgR in hypothalamic supraoptic nucleus neurocytes of rats
- VernacularTitle:视神经横断对大鼠下丘脑视上核神经元Nogo-A/B及NgR表达的影响
- Author:
Xiaolei YIN
;
Rongdi YUAN
;
Jian YE
- Publication Type:Journal Article
- Keywords:
optic nerve injuries;
rats;
supraoptic nucleus;
neurons
- From:
Medical Journal of Chinese People's Liberation Army
2001;0(09):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the influence of bilateral optic nerve transections on the expression of Nogo A/B and NgR in hypothalamic supraoptic nucleus neurocytes of rats in order to elucidate the possible regulatory mechanisms of supraoptic nucleus regeneration.Methods Twelve adult SD rats were randomly assigned into 2 groups(6 each): control group and optic nerve division group(transection of bilateral optic nerves).Sham operation was done in,rats of the control group without transection of sptic nerves.The rats' brains were removed from both groups 7 days after surgery,and then frozen sections of the brains were made.Different antibodies and fluorescent probe dyes were used to label Nogo-A/B and NgR proteins in the hypothalamic supraoptic nucleus neurocytes.And then laser-confocal microscopy was used to observe these proteins.Results No Nogo-A/B and NgR proteins were expressed in neurocytes of hypothalamic supraoptic nucleus in control group.However,Nogo-A/B and NgR proteins were positively expressed around the nuclei of neurocytes in rats of the optic nerve division group 7 days after bilateral optic nerve transection in the said area Conclusions Nogo-A/B and NgR may participate in the regeneration regulation of supraoptic nucleus neurocytes after injury.However,there must be other factors also involved in the regeneration regulation mechanisms.The existence of these factors might explain the strong activity of regeneration of supraoptic nucleus neurocytes after injury even with the existence of the major axon regeneration inhibitor Nogo-A/B and its receptor NgR in the microenvironment.
