Expressions and significance of mesothelin and CA125 in human epithelial ovarian cancer
- VernacularTitle:间皮素与CA125在卵巢癌中的共定位表达及意义
- Author:
Li WANG
;
Na LI
;
Xiaohua WU
- Publication Type:Journal Article
- Keywords:
mesothelin;
CA125;
ovarian neoplasms;
adhesion
- From:Journal of Third Military Medical University
2003;0(16):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the function of mesothelin by analyzing the location,the expressions and the adhesive ability of mesothelin and CA125 in human epithelial ovarian cancer.Methods Mesothelin and CA125 protein expressions and locations in human epithelial ovarian cancer tissues and cell lines were determined by indirect immunofluorescence double maker and Western blot.Cell adhesive ability was detected by EDTA-induced cell detachment.Results Mesothelin and CA125 were specifically located in cytomembrane of human epithelial ovarian cancer cells.Expressions of mesothelin in the SKOV-3 and 3AO cells were weaker than those in the OVCAR-3.SKOV-3 and 3AO cells adhered less effectively to plastic substance and matrigel than OVCAR-3(P
