Effects of urokinase plasminogen activator on metastasis inhibition of gastric cancer cells induced by Parthenolide
- VernacularTitle:尿激酶型纤溶酶原激活剂在小白菊内酯抑制胃癌细胞转移中的作用
- Author:
Lijie ZHAO
;
Yan LI
- Publication Type:Journal Article
- Keywords:
parthenolide;
gastric cancer;
urokinase plasminogen activator
- From:
Chinese Journal of Clinical Pharmacology and Therapeutics
2004;0(08):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To investigate the effect of urokinase plasminogen activator (uPA) on metastasis inhibition of gastric cancer SGC7901cells induced by parthenolide. METHODS: The changes of SGC7901 cells reproductive activity were analyzed by MTT. Light microscope was used to observe the cell morphological changes. The effects of parthenolide on migration and invasion capacity of SGC7901 cells were determined by using matrigel and transwell system. The expression of uPA was detected by Western blot and RT-PCR assays. RESULTS: Parthenolide inhibited the proliferation of SGC7901 cells in a time-and concentration -dependent manner ranging from 100 to 200 ?mol/L. Light microscopy showed the suppressing effect on growth of SGC7901 cells. After exposed to parthenolide, the migration and invasion capacity of SGC7901 cells were significantly decreased, the number of cells gradually decreased through the basement membrance. The Western blot assay showed that the expression of uPA protein declined gradually after exposed to parthenolide for various period of time. CONCLUSION: Parthenolide can inhibit the growth and metastasis activity of SGC7901 cells, and uPA played an important role in the latter process.
