ABSORPTION MECHANISM OF THE ANTIHYPERTENSIVE PEPTIDE VAL-LEU-PRO-VAL-PRO IN CACO-2 CELL MODEL
- VernacularTitle:降血压肽Val-Leu-Pro-Val-Pro在Caco-2细胞模型中的吸收机制
- Author:
Dong LIU
;
Haiyan SUN
;
Lin LEI
;
Shimin LI
- Publication Type:Journal Article
- Keywords:
antihypertensive peptides;
Val-Leu-Pro-Val-Pro;
Caco-2 cell line;
paracellular transport;
efflux
- From:
Acta Nutrimenta Sinica
1956;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the absorption mechanism of the antihypertensive peptide,Val-Leu-Pro-Pro-Val(VLPVP) in Caco-2 cell model. Method Caco-2 cell model was used in vitro to investigate the effects of time,pH,drug concentration,enhancers and inhibitors on the absorption of VLPVP. VLPVP concentration was measured by HPLC. Results The transport rate of VLPVP reached the maximal level at pH7.4,and was positively correlated to transport time and drug concentration. The peptide transporter inhibitors(Gly-Pro,arphamenine A) ,the endocytosis inhibitor(phenyl arsenoxide) didn’t influence the transport of VLPVP,while the paracellular transport enhancer(sodium deoxycholate) ,the inhibitor of multi-drug resistant protein(MK-571) and the energy inhibitor(sodium azide) enhanced it from apical to basolateral side significantly,but the latter two didn’t influence the transport from basolateral to apical side. Conclusion Paracellular diffusion was suggested to have been the main mechanism for the absorption of intact VLPVP and MRP2 had strong eflux effect on the transepithelial transport of VLPVP.
