Determination of Amino Acids in Silkworm Extract by High Performance Liquid Chromatography with Precolumn Derivatization
- VernacularTitle:柱前衍生高效液相色谱法测定蚕蛹提取物中氨基酸含量
- Author:
Weiqing LIANG
;
Kemin WEI
;
Jinbao PU
;
Yongqiang ZHU
;
Junxian ZHENG
;
Yijuan HU
- Publication Type:Journal Article
- Keywords:
Precolumn derivatization;
Silkworm extract;
Amino acid;
RP-HPLC
- From:
Journal of Medical Research
2006;0(08):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To determin the amino acids in silkworm extract by High Performance Liquid Chromatography with Precolumn Derivatization.Methods With the technology of precolumn derivatization, along with high performance liquid DABS-CL and a high-efficiency YMC-Pack ODS-A column(250?4.6mm, 5 ?m), with mobile phase being A:25mol/L KH2PO4 and B: acetonitrile : methyl alcohol =70:30, at flow rate of 1.5 ml/min, peaks were detected at 436nm and column temperature being 35℃.Results All kinds of amino acids have a nice linear relations (r=0.9989~0.9999), precision (RSD
