Determination of Total Glucosides in Paeonia lactifloria Pall from Different Sources by HPLC
- VernacularTitle:高效液相法测定白芍药材中白芍总苷的含量
- Author:
Yijuan HU
;
Jinbao PU
;
Aigui WANG
;
Weiqing LIANG
;
Junxian ZHENG
;
Kemin WEI
- Publication Type:Journal Article
- Keywords:
Paeonia lactifloria pall;
Total glucosides;
Benzoic acid;
Content determination;
HPLC
- From:
Journal of Medical Research
2006;0(08):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To set up a simple and accurate method for the determination of total Glucosides in Paeonia lactifloria (TGP) by HPLC.Methods Benzoic acid being as contiol,the amounts of TGP were obtained from the following formula :TGP( ) = benzoic acid() ?480. 27/ 121. 121. Benzoic acid was determined by HPLC. Chromatographic conditions concluded YMC-Pack ODS-A(5?m,150?4.6mm)column and the mobile phase consisting a mixture of MeOH - 0.05mol?L - 1KH2PO4 - HAC - isopropanol (85:155:4:6). Benzoic acid was detected at 230nm wavelength.Results The calibration curves of Benzoic acid were in good linearity over the range of 0.1~2g(r=1.0000). The average recovery of Benzoic acid was 101.12(n=9,RSD=2.49). Conclusion This method is simple and can be easily repeated.
