Some Visible Properties of T Lymphocyte Activation in Human Umbilical Cord Blood
- VernacularTitle:人脐血T细胞活化的某些可视化特征
- Author:
Xinqing PENG
;
Huaying LIU
;
Yongming LIU
- Publication Type:Journal Article
- Keywords:
T lymphocyte;
Activation;
Cell morphology;
Visualization
- From:
Journal of Medical Research
2006;0(08):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore visible properties of T lymphocyte activation in human umbilical cord blood.Methods T lymphocytes of human umbilical cord blood were cultured in RPMI 1640 complete medium and divided into two groups: the experimental group activated with CD3 McAb(10mg/L)plus PHA and the control group cultured without these reagents. Afcer the cell viability being measured, T cell activation was observed with inverted microscope. And then ultrathin section of the cells sample was prepared tobe observed with transmission electron microscope(TEM).Results Morphologically, activated T cells were obviously bigger, their nuclei clear, and most of divided cells had irregular outline;there were cell colonies to be seen in growing regions. Under TEM, there were more vacuoles (visible property of structure) in typically activated T lymphocytes , chromatic agglutination and nucleoli to be observed.Conclusion Main properties of T cell activation of human umbilical cord blood were chromatic agglutination, more vacuoles in cytoplasm and cell colonies formation.
