The Effect of Combination Use of 1,25(OH)_2D_3 and Celecoxib on Growth and Apoptosis in Breast Cancer Cell Line Hs578T
- VernacularTitle:1,25-二羟维生素D_3联合塞莱昔布对乳腺癌细胞株Hs578T生长及凋亡的影响
- Author:
Xueqi HE
;
Yangyi BAO
;
Xin SUN
;
Gang MENG
;
Qin WANG
- Publication Type:Journal Article
- Keywords:
1,25-dihydroxyvitaminD3;
Celecoxib;
Apotosis;
Cell proliferation
- From:
Journal of Medical Research
2006;0(08):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the effect of combined 1,25-dihydroxyvitamin D31,25(OH)2D3 and celecoxib on growth, call cycle and apoptosis in breast cancer cell line Hs578T.Methods We compared cell numbers by using MTT method , analyzed cell cycle percentage and apoptosis with flow cytometric.Results Both with 1,25(OH)2D3 and celecoxib could inhibite tumov growth,induc apoptosis in a dose-time-dependent manner. comparing with 1,25(OH)2D3 alone,combination wse of the two drugs had a additive effect but was inferior to cececoxib alone. The combination use of 10-8mol/L1,25(OH)2D3 and celecoxib group is more effective than the combination use of 10-7mol/L1,25(OH)2D3 and celecoxib group. Conclusion 1,25(OH)2D3 and celecoxib could be a new drug for the prevention and treatment of breast cancer.
