Result of production of standard Albumin from human plasma.
- Author:
Phan Trung Do
;
Duong Tuan Pham
;
Hien Thi Do
;
Ha Diem Vo
;
Thuy Thi Nguyen
;
Thin Duy Ngo
;
Phuc Hanh Hoang
;
Tri Anh Nguyen
- Publication Type:Journal Article
- Keywords:
Human albumin plasma;
liophilization;
quantitative analysis
- MeSH:
Albumins/ standards;
Plasma;
- From:Journal of Medical Research
2007;52(5):1-6
- CountryViet Nam
- Language:Vietnamese
-
Abstract:
Background: Human albumin was produced and used in many countries. Cohn's technique had been used to precipitate albumin from human plasma. This technique was easy and cheap and the quality of the product was good. In Vietnam, human albumin had to import, but the prices was very expensive. Vietnam was having good plasma in large quantity and high quality. That\u2019s why research on production plasma albumin was essential.\r\n", u"Objectives: This study aimed at using Cohn's technique improved by Drohan and Van - Aken to produce standard albumin from human plasma. Subjects and method: Human plasma detected VIII-factor was used for present study. Plasma \ufffd?albumin was precipitated by ethanol at low temperature and pH. The collected albumins have been liophilizated and storage at 40C. The quality and quantity of Albumin was evaluated by quantitative analysis and protein \ufffd?electrophoresis. Results: The 418g of albumin powder was produced from 16 liters of plasma detected F \ufffd?VIII. The quality of this albumin come up to standard (>95%) and quantity of albumin collected from one liter of this plasma was 26g. Conclusion: In the Vietnamese condition, the technique of Cohn can be used to produce standard albumin for treatment.\r\n", u'
