c-fos expression in cornu dorsale medullae spinalis in crush injury of sciatic nerve in rats
- VernacularTitle:坐骨神经挤压模型大鼠脊髓背角c-fos表达的研究
- Author:
Ming WU
;
Qian CHEN
;
Zhongzhi TANG
- Publication Type:Journal Article
- Keywords:
nerve regeneration;
sciatic nerve;
hyperalgesia
- From:
Medical Journal of Chinese People's Liberation Army
2001;0(08):-
- CountryChina
- Language:Chinese
-
Abstract:
Objectives A rat model sciatic nerve crush injury was reproduced in rat,and behavioral changes,pain threshold,thermal withdrawal latency(TWL)and the changes in c-fos expression in cornu dorsale medullae spinalis were examined,and the relationship between peripheral nerve regeneration and hyperpathia was explored.Methods 40 male Wistar rats weighing 180-200g were randomly divided into sciatic nerve injury group(group A,n=20)and sham-operation group(group B,n=20).The right sciatic nerve of rats in group A was crushed with a hemostat in the middle of the nerve for 30 seconds,until the nerve appeared translucent.The right sciatic nerve of rats in group B was only exposed without crush injury under the same anesthesia.Behavioral response,pain threshold and TWL were assessed on 18,21,24,27,30,33,36 and 39d after operation.c-fos expression was examined immunohistochemically on 21,27,33 and 39d after operation after rats were euthanized.Results The cumulative pain scores of group A were higher than those of group B at every time point(P
