In vitro induction of colon cancer-reactive CTLs with prostatic acid phosphatase
- VernacularTitle:前列腺酸性磷酸酶体外诱导结肠癌特异性CTLs的研究
- Author:
Yi WANG
;
Yan WANG
;
Hongyu ZHAO
- Publication Type:Journal Article
- Keywords:
prostate;
acid phosphatase;
colonic neoplasms;
T lymphocytes,cytotoxic
- From:
Medical Journal of Chinese People's Liberation Army
2001;0(08):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective In this study,we determined whether or not cancer-reactive cytotoxic T-lymphocytes(CTLs)could be induced from peripheral blood mononuclear cells(PBMCs)of colon cancer patients by in vitro stimulation with a PAP peptide.Methods The PBMCs of HLA-A24+ colon cancer patients and healthy donors were stimulated with a PAP213-221 peptide in vitro.The PAP-specific IFN-? was measured by ELISA.The cytotoxicity of CTLs were examined by 51Cr release assay.Results IFN-? produced by the PBMCs of 3 of 5 colon cancer patients and 1 of 8 healthy donors after incubation with PAP213-221 peptide pulsed C1R-A2402 cells were significantly higher then that after incubation with HIV peptide pulsed C1R-A2402 cells(P
