The variety of the mRNA expression of perforin, granzyme B by expanded NK cells with different cytokines from human peripheral blood

VernacularTitle:人外周血NK细胞在细胞因子刺激下扩增后穿孔素、颗粒酶B基因表达的变化
Author: Jian MA ; Xiaohong LI ; Chunji GAO ; Dandan ZHAO
Publication Type:Journal Article
Keywords: NK cells; Perforin; Granzyme B; K562 cells
From: Chinese Journal of Microbiology and Immunology 2009;29(4):336－339-
CountryChina
Language:Chinese
Abstract: Objective To study the relationship between the expression of perforin, granzyme B and the cytotoxicity of NK cells, after NK cells were purified and expanded with different cytokines. Meth-otis It was detected that the mRNA expressions of perforin, ganzyme B from 8 donors, and the cytotoxcity of NK cells to target I(562 cells by competitive qualitative RT-PCR. Results The mRNA expressions of perforin, granzyme B by purified and expanded NK cells with different cytokines were markedly enhanced, and IL-2 +IL-15 group, IL-2 + IL-12 + IL-15 group were significantly higher than others. It is consistent that the relationship between the mRNA expressions of NK cells and the cytotoxicity of NK cells to I(562 cells. Conclusion The mRNA expressions of perforin, granzyme B of NK cells with different cytokines were obviously.