Protective mechanism of metformin in process of human umbilical vein endothelial cell injury induced by palmitic acid in vitro
- VernacularTitle:二甲双胍减轻软脂酸诱导的血管内皮细胞损伤机制探讨
- Author:
Ying CHENG
;
Yiming MU
;
Baoan WANG
;
Changyu PAN
;
Juming LU
- Publication Type:Journal Article
- Keywords:
metformin;
AMP-activated protein kinase;
human umbilical vein endothelial cell;
intercellular adhesion molecule-1;
monocyte chemoattractant protein-1;
nuclear factor kappa B
- From:Journal of Third Military Medical University
2003;0(14):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the protective mechanism of metformin in the process of cultured human umbilical vein endothelial cells (HUVECs) injury induced by palmitic acid (PA). Methods HUVECs were cultured in the media of blank,PA,metformin,or PA combined metformin for 24 h,48 h,72 h respectively. Expressions of monocyte chemoattractant protein-1 (MCP-1) and intercellular adhesion molecule-1 (ICAM-1) were determined by RT-PCR and the expressions of ICAM-1,nuclear factor kappa B p65 (NF-?B p65) and phospho-I?B? was evaluated by Western blotting. Results Significant increase of the expressions of MCP-1 and ICAM-1 was found in PA group compared with in the control group (P
