Celecoxib suppresses xenograft tumor of colon cancer in nude mice
- VernacularTitle:塞来昔布对人结肠癌裸鼠移植瘤的抑制作用及其机制
- Author:
Shipeng ZHAO
;
Fa ZHAO
;
Mingyue XU
;
Xiaochen ZHANG
;
Jianhui CAI
- Publication Type:Journal Article
- Keywords:
colon cancer angiogenesis COX-2 inhibitor survivin
- From:Journal of Third Military Medical University
2003;0(14):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To evaluate the effects of celecoxib on tumor growth,COX-2 and survivin expressions and angiogenesis in nude mice. Methods Xenograft animal model was established by injecting human colon cancer HT-29 cells into the BALB/c nude mice subcutaneously. Fifty mice were randomly divided into 4 groups 16 d after injection:control group,celecoxib group(receiving 25,50,75,100 mg?kg-1?d-1 for 35 d). Tumor volumes were measured every week. The expression level of COX-2,survivin and the microvessel density (MVD) of the xenograft tumor tissues were measured by immunohistochemistry,and mRNA level of VEGF by RT-PCR. Results Celecoxib at dose of 25,50,75 and 100 mg?kg-1?d-1 inhibited the tumor volume by 34.94%,39.20%,53.50%,59.20% respectively,and showed more effective in suppressing the tumor growth than the control group(P
