Effect of poly (ADP-ribose) polymerase inhibitor on liver metastasis of colorectal carcinoma CT26 cell line in mice
- VernacularTitle:PARP抑制剂对小鼠结肠腺癌CT26细胞肝转移的影响
- Author:
Yan QIN
;
Yalan WANG
;
Yuanyuan LI
- Publication Type:Journal Article
- Keywords:
poly(ADP-ribose)polymerase;
5-aminoisoquinolinone;
liver metastasis;
colorectal carcinoma
- From:Journal of Third Military Medical University
2003;0(14):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of poly (ADP-ribose)polymerase (PARP) expression inhibited by 5-AIQ on the liver metastasis in colorectal carcinoma CT26 cell lines. Methods Liver metastasis model of colorectal carcinoma was established by intrasplenic inoculation of colorectal carcinoma CT26 cell in BALB/c mice. Treated by 5-AIQ (100 or 500 ?mol/L),CT26 cells were inoculated to the spleen of the mice,or the rats were first inoculated of CT26 cells,then treated by 5-AIQ (3 or 10 mg/kg) ip. The changes spleen primary carcinoma and liver metastasis carcinoma were observed. The PARP expression in spleen primary carcinoma was detected by Western blotting. Results Significant difference was found in the splenic carcinoma size,the number of liver metastatic nodules,the grading of liver metastatic nodules in the 5-AIQ treated group and control group(P0.05). The PARP expression of spleen primary carcinoma in 5-AIQ treated groups was lower significantly than that in control group (P
