Tyrosine kinase inhibitor A77-1726 inhibiting STAT6 phosphorylation and cfos expression induced by IL-13
- VernacularTitle:酪氨酸激酶抑制剂A77-1726阻断IL-13介导STAT6磷酸化和c-fos表达
- Author:
Xiaoyu SHI
;
Yanchun GONG
;
Wenlin LI
;
Lixia XIONG
;
Ying ZHOU
;
Wei CAI
;
Lin ZHAO
- Publication Type:Journal Article
- Keywords:
tyrosine kinase inhibitor;
interleukin-13;
cell line,Dami;
STAT6;
c-fos
- From:
Chinese Pharmacological Bulletin
2003;0(07):-
- CountryChina
- Language:Chinese
-
Abstract:
Aim To investigate the effects of tyrosine kinase inhibitor A77-1726 on IL-13 induced STAT6 phosphorylation and c-fos expression in Dami cell and to provide novel experimental basis to the clinical application of A77-1726 and the study of IL-13 pathway.Methods Total RNA was extracted from Dami cells incubated with or without IL-13 and A77-1726 respectively for various time points.RT-PCR and agar gel electrophoresis were used to examine the expression of c-fos mRNA.The expression of STAT6 and c-fos proteins was detected by Western blot.A densitometric rel-ative quantitation of PCR and Western blot products was quantitated using Image Quant software.Results STAT6 was phosphorylated by treatment of 100 ?g?L-1 IL-13 in Dami cells.Phosphorylation of STAT6 induced by IL-13 was inhibited by treatment of 50?mol?L-1 A77-1726.The expression of c-fos mRNA was significantly induced by IL-13 treatment in Dami cells(P
