High performance liquid chromatography combined with microwave-assisted extraction and solid phase extraction in determination of mefenacet residues in rice
- VernacularTitle:微波辅助-固相萃取-高效液相色谱法测定稻谷中苯噻草胺残留量
- Author:
Tao WANG
;
Heming MI
;
Yifeng CHAI
;
Guorong FAN
- Publication Type:Journal Article
- Keywords:
microwave-assisted extraction;
solid phase extraction;
high pressure liquid chromatography;
mefenacet;
Oryza sativa
- From:
Academic Journal of Second Military Medical University
2000;0(07):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To establish a method by combining microwave-assisted extraction(MAE),solid phase extraction(SPE)and high performance liquid chromatography(HPLC)for determination of the mefenacet residues in rice.Methods:Acetone and acetontrile(37)were used as extraction solvent.Microwave-assisted extraction was used to extract mefenacet residues in the rice.The extracts were then cleaned up with a Florisil cartridge and then subjected to Hypersil C18 column(5 ?m,4.6 mm?200 mm),with acetonitrilewater(5050,V/V)solution as mobile phase and with a flow rate of 1.0 ml/min;the ultraviolet detection wavelength was at 217 nm.Results:Good linear correlation for mefenacet was found within a concentration range of 0.198-9.900 ?g/ml.The detection limit was 0.039 6 ?g/mL for mefenacet(S/N=2).The average recovery rate of rice hull and brown rice were 90.8%(RSD 1.8%)and 85.6%(RSD 2.5%),respectively.Conclusion:The present method is simple and rapid;it can be used for the determination of mefenacet residues in rice.
