Pax6 expression in rat whisker hair follicle
- VernacularTitle:Pax6基因和蛋白在大鼠触须毛囊中的表达
- Author:
Ke YANG
;
Tian YANG
;
Ziling JIANG
- Publication Type:Journal Article
- Keywords:
paired homebox 6;
hair follicle
- From:Journal of Third Military Medical University
2003;0(13):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the expression pattern of transcription factor,paired homebox 6(Pax6) in rat whisker hair follicle.Methods Whisker hair follicle was collected from 5 7-day-old SD rats.Immunohistochemical assay was carried out to detect Pax6 in the follicle.Pax6 mRNA expressions and its splicosome,Pax6-5a were detected by RT-PCR.Whisker hair follicle was primarily cultured to harvest rat hair follicle bulge cells.Then expressions of Pax6,CD34,?6-integrin,and Ki67 were detected in the obtained cells by immunofluorescent method.Results Pax6 was positively expressed in rat whisker hair follicle.The mRNA expression of Pax6-5a was stronger than that of Pax6.The primarily cultured cells expressed CD34 and ?6-integrin in the membrane.Pax6 was mainly located in the nuclei of the cells with Ki67,and little in the cytoplasm.The proliferative ability of the cells was correlated with the location of Pax6 protein.Conclusion Pax6 expresses in rat whisker hair follicle.Its expression patterns indicate that it may be involved in the formation and function of whisker hair follicle.
