Lipoteichoic acid of Bifidobacterium down-regulates VEGF expressions and inhibits the metastasis of colon carcinoma
- VernacularTitle:双歧杆菌脂磷壁酸对不同大肠癌细胞株转移能力及VEGF表达的影响
- Author:
Yanhua PENG
;
Jianlong LIU
;
Yue WANG
;
Mingfang LIU
;
Shuhui CHEN
- Publication Type:Journal Article
- Keywords:
colon carcinoma line;
lipoteichoic acid;
bifidobacterium;
metastasis;
vascular endothelial growth factor
- From:Journal of Third Military Medical University
2003;0(13):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the effect of lipoteichoic acid(LTA) of Bifidobacterium on the expression of vascular endothelial growth factor(VEGF) and invasion and metastasis of colon carcinoma cell lines.Methods The effects of LTA on adhesion,invasion and metastasis of human colon carcinoma lines LoVo and HT-29 in vitro were examined by MTT colorimetric and transwell chamber.The mRNA and protein expressions of VEGF in the two colon carcinoma cells treated with LTA were detected by RT-PCR and Western blot.Results The adhesion ability decreased in LoVo cells and HT-29 cells after the treatment of LTA(20,50,80 ?g/ml) for 30,60,90,120 min(P
