Effects of Saikosaponin a on TNF-? release and its receptor expression in cultured hippocampal astrocytes induced by pentylenetetrazol
- VernacularTitle:柴胡皂苷a对PTZ诱导大鼠海马星形胶质细胞TNF-?释放及其受体表达的影响
- Author:
Wei XIE
;
Ping KANG
;
Zuowen ZHANG
;
Linlin ZHU
;
Yong BAO
- Publication Type:Journal Article
- Keywords:
Epilepsy;
Astrocyte;
Pentylenetetrazol;
Saikosaponin a;
TNF-?;
TNFR1
- From:
China Journal of Traditional Chinese Medicine and Pharmacy
2005;0(07):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To study e ects of Saikosaponin a(SSa) on tumour necrosis factor-alpha(TNF-?) release and its receptor expression in cultured hippocampal astrocytes induced by pentylenetetrazol(PTZ).Methods:The in vitro cultured primary hippocampal astrocytes were randomly divided into control group(group A),PTZ-induced group(group B)PTZ10mmol/L+SSa groups(group C and group D,the SSa concentrations were 1.25mg/L and 0.625mg/L respectively).The extracellular uid TNF-? level and the expression of tumour necrosis factor receptor type 1(TNFR1) in hippocampal astrocytes were respectively detected by ELISA and Western-blot after PTZ-induced 2 hours.Results:the TNF-? level and TNFR1 expression of group B were signi cantly higher than that of group A,group C and group D(P
