Expressions of inflammatory cytokines in serum and aorta of rats with chronic renal failure
- VernacularTitle:MG-132对慢性肾衰竭大鼠主动脉及循环炎症细胞因子表达的抑制作用
- Author:
Bing FENG
;
Xu YANG
;
Zilin YE
- Publication Type:Journal Article
- Keywords:
kidney failure,chronic;
aorta;
tumor necrosis factor;
peripheral blood mononuclear
- From:
Medical Journal of Chinese People's Liberation Army
2001;0(07):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the changes in the expression of inflammatory cytokines in serum and aorta of rats with chronic renal failure.Methods Animal model of chronic renal failure was established by single nephrectomy and partial ligation of the contralateral renal artery.The rats were randomly divided into three groups(10 each):normal control group(Con),chronic renal failure group(CRF),rats with chronic renal failure and treated with proteasome inhibitor MG-132 group(CRF+M).The protein expression of TNF? was detected by immunohistochemistry and image analysis,the levels of TNF? and IL-1? of peripheral blood mononuclear cells(PBMC) in blood plasma and culture supernatant were detected by ELISA.Results The protein expression of TNF? 4 and 6 months after operation significantly increased in aorta of CRF group compared with that in CON group(10.7?1.3 vs 4.3?1.7,12.6?2.2 vs 4.3?1.1,P
